Pulse-Chase in the Light Microscope

Abstract

A recent publication by Gaietta et al. describes the real-time trafficking of connexin, and through the use of different fluors, researchers can discriminate between “older” and newly synthesized connexins. Goodenough and Postma discuss how the technique permits the visualization of dynamic building and degradation that occurs in subcellular compartments, and how the method can be used to analyze protein fates in real time.