Coming of Age:Anti–Cytokine Therapies

Generation of a phage display library for antibody selection and production. Human B lymphocytes are the source of a repertoire of antibody heavy and light chain gene segments (V_H and V_L) that are inserted in a pair-wise, random manner into a bacteriophage genome. Mature phage expresses the V_H–V_L polypeptides fused to phage coat proteins. Phages that express the desired V_H–V_L polypeptide can be selected and the corresponding antibody genes inserted into a cell line for expression and production of antibody.