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Cannabinoids Biology: The Search for New Therapeutic Targets
Table 3.
Summary of Km and Vmax of Anandamide Transport
| Cell type | Km(μM) | Vmaxa | Reference |
|---|---|---|---|
| a Vmax values were converted to either pmol/min/mg or mol/min/cell where possible to standardize units of anandamide uptake in whole cell preparations. | |||
| Human | |||
| FAAH/HeLa | 18.5 ± 1.6 | 76.7 ± 1.3x10−17 mol/min/cell | (117) |
| Human Hep2 laryngeal carcinoma | 20.9 | 59x10−17 mol/min/cell | (134) |
| HeLa cervical carcinoma | 12.1 ± 2.6 | 43.7 ± 4.6x10−17 mol/min/cell | (117) |
| S217A-FAAH/ HeLa | 12.3 ± 0.5 | 39.7 ± 4.1x10−17 mol/min/cell | (117) |
| PC-3 prostate epithelial | 4.9 ± 0.2 | 33 ± 3 x10−17 mol/min/cell | (135) |
| U937 lymphoma | 0.13 ± 0.01 | 140 ± 15 pmol/min/mg protein | (20) |
| Lymphocytes | 0.13 ± 0.015 | 75 ± 8 pmol/min/mg protein | (136) |
| HUVECs | 0.190 ± 0.010 | 45 ± 3 pmol/min/mg protein | (83) |
| CHP100 neuroblastoma | 0.2 ± 0.02 | 30 ± 3 pmol/min/mg protein | (20) |
| HMC-1 mast cells | 0.2 ± 0.02 | 25 ± 3 pmol/min/mg protein | (137) |
| Platelets | 0.2 ± 0.02 | 22 ± 2 pmol/min/mg protein | (138) |
| CCF-STTG1 astrocytoma cells | 0.6 ± 0.1 | 14.7 ± 1.5 pmol/min/mg protein | (18) |
| Rat | |||
| C6 glioma | 0.7 | 39 x10−17 mol/min/cell | (134) |
| 16 ± 2 | 2800 ± 1000 pmol/min/mg protein | (89) | |
| RBL-2H3 | 11.4 ± 2.3 | 17.5±2.1x10−17 mol/min/cell | (21) |
| 16.4 ± 4.4 | 23.7 ± 2.6 x10−17 mol/min/cell | (117) | |
| 10 ± 2 | 3500 ± 1100 pmol/min/mg protein | (89) | |
| 9.3 ± 3.0 | 11 ± 1.1 x10−17 mol/min/cell | (139) | |
| 33 | 600 pmol/min | (108) | |
| 4.69 ± 0.460 | 2.15 x10−17 mol/min/cell | (22) | |
| Cerebellar granular neurons | 45.0 ± 7.8 | 6500 ± 350 x10−17 mol/min/cell | (140) |
| 41 ± 15 | 6100 ± 400 x10−17 mol/min/cell | (17) | |
| Cortical neurons | 1.2 | 90.9 pmol/min/mg protein | (19) |
| Cortical astrocytes | 0.32 | 171 pmol/min/mg protein | (19) |
| Mouse | |||
| Cerebellar granular neurons | Not available | 7500 ± 900 x10−17 mol/min/cell | (140) |
| Cortical neurons | 1.1 ± 0.1 | 151 ± 8 pmol/min/mg protein | (88) |
| Cortical neurons FAAH−/− | 1.3 ± 0.1 | 157 ± 15 pmol/min/mg protein | (88) |
| N18 neuroblastoma | 1.8 | 174 x10−17 mol/min/cell | (134) |
| Neuro-2a neuroblastoma | 10 ± 3.8 | 13 ± 1.7 x10−17 mol/min/cell | (139) |
| FAAH+/+ brain synaptosomes | 7 ± 1 | 110 ± 20 pmol/min/mg protein | (89) |
| FAAH−/− brain synaptosomes | 5 ± 1 | 50 ± 10 pmol/min/mg protein | (89) |
| Sertoli cells (4d old) | 0.12 ± 0.016 | 86 ± 9 pmol/min/mg protein | (141) |
| Sertoli cells (16d old) | 0.12 ± 0.016 | 58 ± 6 pmol/min/mg protein | (141) |
