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HETEROCYCLES
An International Journal for Reviews and Communications in Heterocyclic ChemistryWeb Edition ISSN: 1881-0942
Published online by The Japan Institute of Heterocyclic Chemistry
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Received, 29th July, 2008, Accepted, 9th September, 2008, Published online, 11th September, 2008.
DOI: 10.3987/COM-08-S(F)72
■ A Convenient Synthetic Method for Fluorine-containing 4-Alkoxy-dihydrobenzo[b][1,4]diazepinols and 3H-Benzo[b][1,4]diazepines by the Reaction of β-Trifluoroacetylketene Acetals with 1,2-Phenylenediamines
Norio Ota, Etsuji Okada,* Naoya Terai, Tomomi Miyamura, Dai Shibata, and Tsuneaki Sakai
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan
Abstract
β-Trifluoroacetylketene dialkyl acetals (3 and 5) reacted easily with various 1,2-phenylenediamines to give novel 4-alkoxy-2-trifluoromethyl-2,3- dihydro-1H-benzo[b][1,4]diazepinols (6 and 8) in moderate to high yields. Dehydration of 6 and 8 proceeded thermally under reduced pressure to afford the corresponding fluorine-containing 3H-benzo[b][1,4]diazepines (7 and 9).INTRODUCTION
Benzo[b][1,4]diazepines have attracted much attention as an important class of heterocycles in the field of medicinal and agricultural chemistries.1 These compounds are widely used as powerful agents, especially for the antipsychotic therapy.2 Very recently, we have reported the facile synthesis of novel fluorine-containing dihydrobenzo[b][1,4]diazepinols (2),3 some of which showed remarkable antineoplastic efficacy,4 by the reaction of β,β-bis(trifluoroacetyl)vinyl ethers (1) with various 1,2-phenylenediamines (Scheme 1). In our previous studies, it was found that ketene dithioacetals5 and orthoacetates6 reacted with trifluoroacetic anhydride quite easily to afford the corresponding β-trifluoroacetylated ketene S,S- and O,O-acetals, respectively, and that these acylated compounds cleanly underwent nucleophilic S-N and O-N exchange reactions7,8 with aliphatic and aromatic amines to give β-trifluoroacetylated ketene S,N-, O,N-, and N,N-acetals. Thereafter, these β-trifluoroacetyl-
ketene acetals were found to be convenient building blocks which are applicable to the syntheses of a variety of novel fluorine-containing heterocyclic compounds by the reactions with bifunctional nucleophiles. For instance, fluorine-containing isoxazolines, 1H-pyrazolines, and imidazolines were easily obtained by the reactions with hydroxylamine, hydrazines, and 1,2-ethylenediamine, respectively.9,10 Additionally, Reddy et al. reported the interesting reaction of β-trifluoroacetylketene acetal (3) with 4,5-dimethyl-1,2-phenylenediamine under microwave irradiation to give solely benzimidazoles (4) without any formation of benzodiazepines (Scheme 2).11 These studies and our
continuing interest in the synthesis of trifluoromethyl-containing benzodiazepines, a new potential pharmacophore of antineoplastic efficacy, prompted us to explore the feasibility of the benzodiazepine- ring construction by the non-microwave assisted cyclization reaction of β-trifluoroacetylketene acetals with 1,2-phenylenediamines. In this paper we wish to report the annulation reaction of β-trifluoroacetylketene acetals (3 and 5) with various 1,2-phenylenediamines under very mild conditions without microwave irradiation to afford the desired fluorine-containing 2,3-dihydro-1H-benzo[b][1,4]diazepinols (6 and 8) having an alkoxy group at the C-4 position. In addition, the thermally induced dehydration of 6 and 8 under reduced pressure leading to the formation of the corresponding 2-alkoxy-3H-benzo[b][1,4]diazepines (7 and 9) is presented.
RESULTS AND DISCUSSION
β-Trifluoroacetylketene dimethyl acetal (5) was easily prepared in 92% yield by the acylation of trimethyl orthoacetate with trifluoroacetic anhydride according to our method reported previously.6,12 The results of the annulation reaction of 5 with 1,2-phenylenediamines are depicted in Scheme 3 and summarized in Table 1. Reaction of 5 with slightly excess amounts of 1,2-phenylenediamine readily occurred at room
temperature in acetonitrile to give the desired 4-methoxy-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol (6a) in 68% yield with the formation of its dehydrated product, 2-methoxy-4-trifluoromethyl-3H-benzo[b][1,4]diazepine (7a), in 5% yield (Entry 1). Similarly, 4,5-dimethyl- and 4,5-dichloro-1,2-phenylenediamines reacted with 5 to afford the corresponding dihydrobenzodiazepinols (6b,c) — benzodiazepines (7b,c) mixtures in high combined yields (Entries 2 and 3). The annulation with unsymmetrical 1,2-phenylenediamines such as 4-benzoyl- and 4-nitro-1,2-phenylenediamines also proceeded cleanly to afford the mixtures of the four components, the two regioisomers of dihydrobenzodiazepinols (6d,d’ and 6e,e’) and those of benzodiazepines (7d,d’ and 7e,e’), in excellent combined yields (Entries 4 and 5). Although the attempted separation of mixtures of the two regioisomers was unsuccessful, all of the separation of the mixtures into 6a-e’ and 7a-e’ was easily performed by column chromatography.
These results of our approaches on the reactions of 5 with 1,2-phenylenediamines show sharp contrast with those reported by Reddy et al (see Scheme 2).11 As shown in Scheme 3, dihydrobenzodiazepinols (6) were obtained predominantly, together with the small amounts of the corresponding benzodiazepines (7) under very mild conditions in the absence of microwave irradiation in acetonitrile without any formation of the corresponding benzimidazoles (4). To verify no difference in reactivities between dimethyl derivative (5) and diethyl one (3), we then examined the reaction of 3 with 4,5-dimethyl-1,2-phenylenediamine, in which 4-ethoxy derivative of 2,3-dihydro-1H-benzo[b][1,4]diazepinols (8) was obtained solely in 66% yield without corresponding benzimidazoles (4) as depicted in Scheme 4.
Next we attempted the acid-catalyzed dehydration of 2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ols (6a-e’ and 8) to 3H-benzo[b][1,4]diazepines (7a-e’ and 9), but this resulted in failure to give complex mixtures. So, we tried to carry out the present dehydration with the use of Kugelrohr distillation apparatus, namely, by heating under reduced pressure, as depicted in Scheme 5 and summarized in Table 2. All dihydrobenzodiazepinols (6a-e’ and 8) was thoroughly dehydrated at 110-150 °C under reduced pressure (3 mmHg) within 1.5 h to provide the corresponding benzodiazepines (7a-e’ and 9) in high yields except for the cases of 7a and 7e,e’ with formation of large amounts of decomposition products.
In summary, we have developed a facile and convenient synthetic method for 4-alkoxy-2-trifluoromethyl-
2,3-dihydro-1H-benzo[b][1,4]diazepinols (6 and 8), which are not easily obtained by other methods. Moreover, the thermal dehydration of 6 and 8 was successfully carried out under reduced pressure to give 2-alkoxy-4-trifluoromethyl-3H-benzo[b][1,4]diazepines (7 and 9). Evaluation of biological activities, especially of antineoplastic efficacy, is now under way for novel fluorine-containing benzodiazepines (6-9).
EXPERIMENTAL
Mps were determined on an electrothermal digital melting point apparatus and are uncorrected. The 1H NMR spectra were recorded on a Bruker AVANCE500 spectrometer using TMS as an internal standard. IR spectra were taken with a PerkinElmer Spectrum ONE FT-IR spectrometer. Microanalyses were taken with a YANACO CHN-Corder MT-5 analyzer.
Synthesis of 1,1,1-trifluoro-4,4-dimethoxybut-3-en-2-one (5).12
To an ice-cooled stirred solution of 1,1,1-trimethoxyethane (4.81 g, 40 mmol) and pyridine (6.33 g, 80 mmol) in CHCl3 (40 mL) was added dropwise trifluoroacetic anhydride (16.80 g, 80 mmol) and the mixture was stirred for 3 h at rt. Then, CH2Cl2 (100 mL) was added and the whole mixture was washed with 10 % aq. Na2CO3 (50 mL) and with water (50 mL), and dried over Na2SO4. The solvent and pyridine was removed in vacuo to give 5 (6.76 g, 92%). 5: mp 67-68 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 4.98 (s, 1H, =CH), 3.98 (s, 3H, CH3), 3.92 (s, 3H, CH3); IR (KBr): 1675 (C=O) cm-1. Anal. Calcd for C6H7F3O3: C, 39.14; H, 3.83. Found: C, 39.16; H, 3.82.
General procedure for the synthesis of 4-methoxy-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ols (6a-e’) and 4-methoxy-2-trifluoromethyl-3H-benzo[b][1,4]diazepines (7a-e’).
To a solution of 5 (184 mg, 1.0 mmol) in MeCN (4 mL) was added 1,2-phenylenediamines (1.1 mmol) and the mixture was stirred for 1 h at rt. The solvent was removed under reduced pressure and the crude mixture was chromatographed on silica gel column using n-hexane-EtOAc (9:1) as eluent to give 6a-e’ and 7a-e’. In the case with 4-benzoyl-1,2-phenylenediamine, a mixture of 6d and 6d’ and a mixture of 7d and 7d’ were eluted respectively. Similarly, a mixture of 6e and 6e’ and a mixture of 7e and 7e’ were eluted respectively in the case with 4-nitro-1,2-phenylenediamine.
4-Methoxy-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol (6a): mp 126-127 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 7.10-6.81 (m, 4H, Harom), 4.11 (s, 1H, OH or NH), 3.91 (s, 3H, OCH3), 3.27 (s, 1H, OH or NH), 2.82 (d, 1H, J = 14.0 Hz, CH2), 2.61 (d, 1H, J = 14.0 Hz, CH2); IR (KBr): 3326, 3112, 1652 cm-1. Anal. Calcd for C11H11F3N2O2: C, 50.77; H, 4.26; N, 10.77. Found: C, 50.72; H, 4.43; N, 10.65.
4-Methoxy-7,8-dimethyl-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol (6b): mp 122-123 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 6.89 (s, 1H, Harom), 6.62 (s, 1H, Harom), 4.05 (s, 1H, OH or NH), 3.88 (s, 3H, OCH3), 3.14 (s, 1H, OH or NH), 2.81 (d, 1H, J = 14.0 Hz, CH2), 2.59 (d, 1H, J = 14.0 Hz, CH2), 2.20 (s, 3H, CH3), 2.19 (s, 3H, CH3); IR (KBr): 3321, 3082, 1652 cm-1. Anal. Calcd for C13H15F3N2O2: C, 54.16; H, 5.24; N, 9.72. Found: C, 54.10; H, 5.01; N, 9.47.
7,8-Dichloro-4-methoxy-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol (6c): mp 117-118 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 7.21 (s, 1H, Harom), 6.97 (s, 1H, Harom), 4.21 (s, 1H, OH or NH), 3.89 (s, 3H, OCH3), 3.17 (s, 1H, OH or NH), 2.84 (d, 1H, J = 14.0 Hz, CH2), 2.63 (d, 1H, J = 14.0 Hz, CH2); IR (KBr): 3315, 3071, 1648 cm-1. Anal. Calcd for C11H9Cl2F3N2O2: C, 40.14; H, 2.76; N, 8.51. Found: C, 40.28; H, 2.74; N, 8.40.
A mixture of (2-hydroxy-4-methoxy-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-8-yl)phenylmethanone (6d) and (2-hydroxy-4-methoxy-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-7-yl)phenylmethanone (6d’): 1H NMR (CDCl3): δ 7.74-6.82 (m, 8H, Harom), 4.61 (s, 1H, OH or NH), 4.50 (s, 1H, OH or NH), 3.92, 3.86 (s, 3H, OCH3), 2.89 (d, 1H, J = 14.0 Hz, CH2), 2.73 (d, 1H, J = 14.0 Hz, CH2); IR (KBr): 3372, 3324, 1662, 1646 cm-1. Anal. Calcd for C18H15F3N2O3: C, 59.34; H, 4.15; N, 7.69. Found: C, 59.49; H, 4.41; N, 8.02.
A mixture of 4-methoxy-8-nitro-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol (6e) and 4-methoxy-7-nitro-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol (6e’): 1H NMR (CDCl3 + CD3CN): δ 8.20-7.73 (m, 2H, Harom), 7.23-7.07 (m, 1H, Harom), 5.60 (s, 1H, NH or OH), 5.50 (s, 1H, NH or OH), 4.00, 3.90 (s, 3H, OCH3), 2.89 (d, 1H, J = 14.0 Hz, CH2), 2.73 (d, 1H, J = 14.0 Hz, CH2); IR (KBr): 3487, 3300, 3170, 1649 cm-1. Anal. Calcd for C11H10F3N3O4: C, 43.29; H, 3.30; N, 13.77. Found: C, 43.38; H, 3.33; N, 13.65.
2-Methoxy-4-trifluoromethyl-3H-benzo[b][1,4]diazepine (7a): bp (oven temperature) 110 °C / 3 mmHg; 1H NMR (CDCl3): δ 7.63-7.13 (m, 4H, Harom), 3.87 (s, 3H, OCH3), 3.10 (s, 2H, CH2); IR (KBr): 1646 cm-1. Anal. Calcd for C11H9F3N2O: C, 54.55; H, 3.75; N, 11.57. Found: C, 54.79; H, 3.71; N, 11.37.
2-Methoxy-7,8-dimethyl-4-trifluoromethyl-3H-benzo[b][1,4]diazepine (7b): bp (oven temperature) 120 °C / 3 mmHg; 1H NMR (CDCl3): δ 7.32 (s, 1H, Harom), 7.16 (s, 1H, Harom), 3.90 (s, 3H, OCH3), 3.08 (s, 2H, CH2), 2.30 (s, 6H, CH3); IR (KBr): 1648 cm-1. Anal. Calcd for C13H13F3N2O: C, 57.78; H, 4.85; N, 10.37. Found: C, 57.96; H, 4.94; N, 10.10.
7,8-Dichloro-2-methoxy-4-trifluoromethyl-3H-benzo[b][1,4]diazepine (7c): mp 98-99 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 7.66 (s, 1H, Harom), 7.49 (s, 1H, Harom), 3.93 (s, 3H, OCH3), 3.16 (s, 2H, CH2); IR (KBr): 1648 cm-1. Anal. Calcd for C11H7Cl2F3N2O: C, 42.47; H, 2.27; N, 9.01. Found: C, 42.87; H, 2.36; N, 8.68.
A mixture of (2-methoxy-4-trifluoromethyl-3H-benzo[b][1,4]diazepin-7-yl)phenylmethanone (7d) and (4-methoxy-2-trifluoromethyl-3H-benzo[b][1,4]diazepin-7-yl)phenylmethanone (7d’): bp (oven temperature) 140 °C / 3 mmHg; 1H NMR (CD3CN): δ 8.07-7.35 (m, 8H, Harom), 3.94, 3.91 (s, 3H, OCH3), 3.29 (s, 2H, CH2); IR (KBr): 1657, 1642 cm-1. Anal. Calcd for C18H13F3N2O2: C, 62.43; H, 3.78; N, 8.09. Found: C, 62.39; H, 3.98; N, 7.93.
A mixture of 2-methoxy-7-nitro-4-trifluoromethyl-3H-benzo[b][1,4]diazepine (7e) and 4-methoxy-7-nitro-2-trifluoromethyl-3H-benzo[b][1,4]diazepine (7e’): bp (oven temperature) 150 °C / 3 mmHg; 1H NMR (CDCl3): δ 8.27-8.00 (m, 2H, Harom), 7.73-7.57 (m, 1H, Harom), 3.81 (br s, 3H, OCH3), 3.24 (s, 2H, CH2); IR (KBr): 1646 cm-1. Anal. Calcd for C11H8F3N3O3: C, 46.00; H, 2.81; N, 14.63. Found: C, 46.02; H, 2.98; N, 14.44.
Synthesis of 4-ethoxy-7,8-dimethyl-2-trifluoromethyl-2,3-dihydro-1H-benzo[b][1,4]diazepin-2-ol(8).
To a solution of 36 (214mg, 1.0 mmol) in MeCN (4 mL) was added 4,5-dimethyl-1,2-phenylenediamine (150 mg, 1.1 mmol) and the mixture was stirred for 1 h at room temperature. The solvent was removed under reduced pressure and the crude mixture was chromatographed on silica gel column using n-hexane-EtOAc (4:1) as eluent to give 8 (200 mg, 66%). 8: mp 117-118 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 6.79 (s, 1H, Harom), 6.66 (s, 1H, Harom), 4.35 (s, 1H, OH or NH), 4.24 (q, 2H, J = 7.0 Hz, CH2CH3), 4.12 (s, 1H, OH or NH), 2.71 (d, 1H, J = 14.0 Hz, CH2), 2.48 (d, 1H, J = 14.0 Hz, CH2), 2.16 (s, 6H, CH3), 1.30 (t, 3H, J = 7.0 Hz, CH2CH3); IR (KBr): 3332, 3095, 1648 cm-1. Anal. Calcd for C14H17F3N2O2: C, 55.62; H, 5.67; N, 9.27. Found: C, 55.66; H, 5.66; N, 9.22.
General procedure for the dehydration of dihydrobenzodiazepinols (6 and 8) to benzodiazepines (7 and 9).
The dehydration of 6 and 8 was successfully carried out with the use of Kugelrohr distillation apparatus. The conditions are as follows: oven temperature, see Table 2; heating time, 1.5 h; reduced pressure, 3 mmHg.
2-Ethoxy-7,8-dimethyl-4-trifluoromethyl-3H-benzo[b][1,4]diazepine (9): mp 82-83 °C (n-hexane-EtOAc); 1H NMR (CDCl3): δ 7.26 (s, 1H, Harom), 7.09 (s, 1H, Harom), 4.30 (q, 2H, J = 7.0 Hz, CH2CH3), 3.07 (s, 2H, CH2), 2.31 (s, 3H, CH3), 2.30 (s, 3H, CH3), 1.32 (t, 3H, J = 7.0 Hz, CH2CH3); IR (KBr): 1642, 1615 cm-1. Anal. Calcd for C14H15F3N2O: C, 59.15; H, 5.32; N, 9.85. Found: C, 59.20; H, 5.36; N, 10.04.
This paper is dedicated to Professor Emeritus Keiichiro Fukumoto on the occasion of his 75th birthday.
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