| ORIGINAL ARTICLE |
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| Year : 2012 | Volume
: 18
| Issue : 1 | Page : 43-46 |
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Prevalence of methylenetetrahydrofolate reductase C677T polymorphism in eastern Uttar Pradesh
Vandana Rai, Upendra Yadav, Pradeep Kumar
Department of Biotechnology, Human Molecular Genetics Laboratory, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India
Correspondence Address:
Vandana Rai
Department of Biotechnology, VBS Purvanchal University, Jaunpur - 22001, Uttar Pradesh
India
 Source of Support: University Grants Commission, New Delhi (grant No. 32-548/2006(SR)) and Department of Biotechnology (No BT/PR98887/SPD/11/1028/2007), Conflict of Interest: None  | 6 |
DOI: 10.4103/0971-6866.96645
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Aim: This study was aimed to evaluate the 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677T mutation in eastern Uttar Pradesh population.
Materials and Methods: Polymerase chain reaction (PCR) using specific primers followed by amplicon digestion by Hinf I restriction enzyme was used for MTHFR C677T polymorphism analysis. Total 250 subjects were analyzed.
Results: The CC genotype was found in 192 subjects, followed by CT in 56 subjects and TT in 2 subject. Genotype frequencies of CC, CT and TT were 0.768, 0.224 and 0.008, respectively. The frequency of C allele was found to be 0.88 and that of T allele was 0.12.
Conclusion: It is evident from the results of the present study that the percentage of homozygous genotype (CC) is highest in the target population. |
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