| ORIGINAL ARTICLE |
|
| Year : 2013 | Volume
: 19
| Issue : 2 | Page : 183-187 |
|
|
Analysis of methionine synthase reductase polymorphism (A66G) in Indian Muslim population
Vandana Rai, Upendra Yadav, Pradeep Kumar, Sushil Kumar Yadav
Human Molecular Genetics Laboratory, Department of Biotechnology, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India
Correspondence Address:
Vandana Rai
Human Molecular Genetics Laboratory, Department of Biotechnology, VBS Purvanchal University, Jaunpur - 222 001, Uttar Pradesh
India
 Source of Support: None, Conflict of Interest: None  | 3 |
DOI: 10.4103/0971-6866.116123
|
|
|
Background and Objectives: Methionine synthase reductase (MTRR) is a vital enzyme of homocysteine/methionine metabolic pathway and is required for the conversion of inactive form of methionine synthase (MTR) to its active form. A clinically important allelic variant of MTRR A66G, with less enzymatic activity is reported with worldwide prevalence rate of ~ 30%. The present study was designed to determine the frequency of MTRR A66G polymorphism in rural Sunni Muslim population of Eastern Uttar Pradesh.
Materials and Methods: Total 56 subjects were analyzed for MTRR A66G polymorphism. A66G mutation analysis was carried out according to the polymerase chain reaction-restriction fragment length polymorphism method of Wilson et al. [1] amplification with MTRR specific primers followed by amplicon digestion with NdeI enzyme was used for the identification of different MTRR genotypes in subjects.
Results and Discussion: The AA genotype was found in 5 subjects, AG in 23 subjects, and GG genotype in 28 subjects. Genotype frequencies of AA, AG, and GG were 0.089, 0.41, and 0.5 respectively. The allele frequency of A allele was found to be 0.298 and G allele was 0.705.
Conclusion: It is evident from the present study that the percentage of homozygous genotype GG and frequency of G allele is high in the target Muslim population. |
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
