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Distribution and genotype frequency of the C1431T and pro12ala polymorphisms of the peroxisome proliferator activator receptor gamma gene in an Iranian population
Hassan Rooki1, Monir-Sadat Haerian1, Pedram Azimzadeh1, Mahmoud Ebrahimi2, Reza Mirhafez2, Gordon Ferns3, Majid Ghayour-Mobarhan2, Mohammad-Reza Zali1
1 Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Cardiovascular Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 Institute for Science and Technology in Medicine, University of Keele, Guy Hilton Research Centre, Thornburrow Drive, Stok on Trent, Staffordshire, United Kingdom
Correspondence Address:
Mohammad-Reza Zali
7th Floor, Taleghani Hospital, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Velenjak, Shahid Aerabi Street, Yemen Street, Tehran
Iran
 Source of Support: This work was financially supported as a PhD thesis
(no. 316) by Gastroenterology and Liver Disease Research Center, Shahid
Beheshti University of Medical Sciences, Tehran, Iran, Conflict of Interest: None
DOI: 10.4103/0971-6866.124370
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Background: Peroxisome proliferator activator receptor gamma (PPARγ) is a nuclear transcription factor regulating multiple genes involved in cell growth, differentiation, carbohydrate and lipid metabolism and energy production. Several genetic variations in the PPARγ gene have been identified to be associated with diabetes, obesity, dyslipidemia, insulin resistance, metabolic syndrome and coronary artery disease. The present study was designed to explore the distribution of two common single nucleotide polymorphisms of the PPARγ gene (C1431T and Pro12Ala) in an Iranian population.
Materials and Methods: Genotype frequencies for these two polymorphisms were compared for 160 healthy Iranian individuals with reports from other populations. The Genotyping was performed using real-time polymerase chain reaction.
Results: The genotype distribution of the C1431T PPARγ polymorphism was 0.869 for the CC genotype, 0.119 for the CT genotype and 0.013 for uncommon TT genotype. Allelic frequencies were 0.93 for C and 0.07 for T allele respectively. For the Pro12Ala polymorphism of PPARγ gene, genotypic distributions and allelic frequencies were, 0.813 for CC, 0.181 for CG and 0.06 for GG and 0.903 for C and 0.097 for G respectively. Allelic and genotypic frequencies for both polymorphisms of PPARγ gene were in Hardy-Weinberg equilibrium.
Conclusions: Iran is a country with an ethnically diverse population and a comparison of allelic and genotypic frequencies of PPARγ C1431T and Pro12Ala polymorphisms between our population and others showed significant differences. |
