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Figure 2: RFLP analysis for the C677T mutation on 198-bp MTHFR PCR products with Hinf I. Wild-type homozygous remains uncut after Hinf I digestion gives one band, mutant homozygous gives two bands (175-bp and 23-bp), and heterozygous gives three bands(198-bp,175-bp, and 23-bp).The figure shows normal CC (lanes: 2, 5, and 7), heterozygous CT (lanes: 3 and 6), and homozygous mutant TT (lane: 4) genotypes

Figure 2: RFLP analysis for the C677T mutation on 198-bp MTHFR PCR products with Hinf I. Wild-type homozygous remains uncut after Hinf I digestion gives one band, mutant homozygous gives two bands (175-bp and 23-bp), and heterozygous gives three bands(198-bp,175-bp, and 23-bp).The figure shows normal CC (lanes: 2, 5, and 7), heterozygous CT (lanes: 3 and 6), and homozygous mutant TT (lane: 4) genotypes