Figure 2: Spectrum Aqua filter set fluorhopore-labeled alpha satellite DNA 18 probe, Spectrum Green probelabeled alpha satellite DNA X probe and Spectrum Orange probe-labeled alpha satellite DNA Y probe. We used DAPI/Orange/Green Filter set for enumeration to CEP X Spectrum Green/CEP Y Spectrum Orange. Fluorescent in situ hybridization (FISH) result showed that there were two chromosomes X and one Y chromosomes