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Figure 1: Polymerase chain reaction amplification of Fragile X mental retardation gene 5' region using a serial dilution of genomic DNA in the presence of betaine (1.0 M) and dimethyl sulfoxide (5%). Lane 1: 100 bp ladder, Lane 2: 10 ng, Lane 3: 15 ng, Lane 4: 25 ng, Lane 5: Negative control (without DNA). The cycle conditions: Initial denaturation 94°C for 15 s followed by 40 cycles of denaturation at 94°C for 15 s, annealing at 65°C for 2 min, and extension at 68°C for 5 min for 40 cycles

Figure 1: Polymerase chain reaction amplification of Fragile X mental retardation gene 5' region using a serial dilution of genomic DNA in the presence of betaine (1.0 M) and dimethyl sulfoxide (5%). Lane 1: 100 bp ladder, Lane 2: 10 ng, Lane 3: 15 ng, Lane 4: 25 ng, Lane 5: Negative control (without DNA). The cycle conditions: Initial denaturation 94°C for 15 s followed by 40 cycles of denaturation at 94°C for 15 s, annealing at 65°C for 2 min, and extension at 68°C for 5 min for 40 cycles