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Year : 2014  |  Volume : 2  |  Issue : 1  |  Page : 32-37

Real-time cell analysis of cytotoxicity of orthodontic cements on gingival fibroblasts

1 Department of Orthodontics, Faculty of Dentistry, Inonu University, Malatya, Turkey
2 Research Center of Faculty of Dentistry, Selcuk University, Konya, Turkey
3 Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkey

Correspondence Address:
Ebubekir Toy
Department of Orthodontics, Faculty of Dentistry, Inonu University, 44280 Malatya
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2321-3825.125922

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Introduction: To evaluate the cytotoxicity of four different orthodontic cement materials using the real-time xCELLigence system. Materials and Methods: Four orthodontic glass ionomer cements (GICs) were selected for this study, namely: GC Fuji (GC Cooperation), Ultra Band Lok (Reliance), Multi Cure (3M Unitek), and Meron (Voco). Ten test cylinders (measuring 5 Χ 2 mm) of each material were fabricated, making a total of 40 cylinders. The samples were incubated in Dulbecco modified Eagle medium (DMEM) culture medium for 72 hours. Human gingival fibroblasts (HGFs) were maintained with DMEM containing 10% fetal bovine serum. A real-time cell analyzer (RT-CES, xCELLigence) was used to evaluate cell survival. After seeding 200 μL of the cell suspensions into the wells (10,000 cells/well), gingival fibroblasts were treated with bioactive components released from cement materials and were monitored every 15 minutes for a period of 88 hours. For proliferation experiments, the statistical analyses used were one-way analysis of variance (ANOVA) and Tukey-Kramer multiple comparisons tests. Results: When the data were evaluated at 24 and 48 hrs, all tested materials showed statistically significant decreases in HGF cell index compared to the control group (P < 0.001). Conclusion: According to the results of this study, all tested cements were found to have cytotoxic effects to the HGFs.

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