Developing a New Generation of TNFα Antagonists for the Treatment of Rheumatoid Arthritis

Standard method used to generate murine MAbs. Splenocytes from immunized mice are fused with mouse myeloma cells to generate antibody-secreting, immortalized hybridoma cells. The monoclonal antibody produced by individual hybridoma cell clones are then screened for optimal antigen binding, as defined by the complimentarity-determining regions (CDR) on the Fab portion of the antibody, as well as for the desired isotype, as defined by the Fc portion of the antibody.