| ORIGINAL ARTICLE |
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| Year : 2013 | Volume
: 19
| Issue : 2 | Page : 219-232 |
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Human leukocyte antigen alleles, genotypes and haplotypes frequencies in renal transplant donors and recipients from West Central India
Jaina S Patel1, Manisha M Patel1, Prakash G Koringa1, Tejas M Shah1, Amrutlal K Patel1, Ajai K Tripathi1, Anila Mathew2, Mohan M Rajapurkar2, Chaitanya G Joshi1
1 Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India
2 Department of Nephrology, Muljibhai Patel Urological Hospital, Nadiad, Gujarat, India
Correspondence Address:
Chaitanya G Joshi
Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand - 388 001, Gujarat
India
 Source of Support: None, Conflict of Interest: None  | 1 |
DOI: 10.4103/0971-6866.116122
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Background: Human leukocyte antigen (HLA) is comprised of a highly polymorphic set of genes which determines the histocompatibility of organ transplantation. The present study was undertaken to identify HLA class I and class II allele, genotype and haplotype frequencies in renal transplant recipients and donors from West Central India.
Materials and Methods: HLA typing was carried out using Polymerase Chain Reaction-Sequence Specific Primer in 552 live related and unrelated renal transplant recipients and donors.
Results: The most frequent HLA class I and class II alleles and their frequencies in recipients were HLA-A*01 (0.1685) and A*02 (0.1649), HLA-B*35 (0.1322), and HLA-DR beta 1 (DRB 1)*15 (0.2192), whereas in donors, these were HLA-A*02 (0.1848) and A*01 (0.1667), HLA-B*35 (0.1359), and HLA-DRB1*15 (0.2409). The two-locus haplotype statistical analysis revealed HLA-A*02-B61 as the most common haplotype with the frequency of 0.0487 and 0.0510 in recipients and donors, respectively. Further, among the three locus haplotypes HLA-A*33-B*44-DRB1*07 and HLA-A*02-B*61-DRB1*15 were the most common haplotypes with frequencies 0.0362 and 0.0326, respectively in recipients and 0.0236 and 0.0323, respectively in donors. Genotype frequency revealed a high prevalence of genotype HLA-A*02/A*24 in recipients (0.058) compared to donors (0.0109) whereas low prevalence of HLA-A*01/A*02 in recipients (0.0435) than in donors (0.0797). The phylogenetic and principal component analysis of HLA allele and haplotype frequency distribution revealed genetic similarities of various ethnic groups. Further, case control analysis provides preliminary evidence of association of HLA-A genotype ( P < 0.05) with renal failure.
Conclusion: This study will be helpful in suitable donor search besides providing valuable information for population genetics and HLA disease association analysis. |
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